Extended Data Fig. 4: Characterization of mouse 2D culture. | Nature Cell Biology

Extended Data Fig. 4: Characterization of mouse 2D culture.

From: Dissection of gastric homeostasis in vivo facilitates permanent capture of isthmus-like stem cells in vitro

Extended Data Fig. 4: Characterization of mouse 2D culture.The alternative text for this image may have been generated using AI.

(a) Entropy score on 2D culture embedded as shown in Fig. 3g. (b) Joint UMAP embedding of combined corpus and antrum 2D cultures with primary corpus and antrum cells showing expression levels of isthmus markers, Mki67 and Birc5, base/neck markers, Gkn3 and Aqp5, and pit markers Tff1 and Klf4. (c) MA plot comparing corpus 2D-cultured cells with antrum 2D-cultured cells using bulk RNA-sequencing counts. Genes in red were identified as being significantly differentially expressed based on a cutoff of FC > 2 and FDR < 0.05 (n = 2, biologically independent samples). Genes highlighted in green have been shown to discriminate corpus from antrum in vivo. (d) Joint UMAP embedding of primary corpus or antrum cells showing expression levels corpus-specific DEG Esrrg and antrum-specific DEG Nkx6-3. (e) Representative expression analysis of Gif in mouse antrum 2D culture by immunofluorescence, scale bar, 100 μm.

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