Extended Data Fig. 5: Characterization of human 2D culture. | Nature Cell Biology

Extended Data Fig. 5: Characterization of human 2D culture.

From: Dissection of gastric homeostasis in vivo facilitates permanent capture of isthmus-like stem cells in vitro

Extended Data Fig. 5: Characterization of human 2D culture.The alternative text for this image may have been generated using AI.

(a) Section of human stomach that was used to isolate and expand glandular stomach cells using 2D media. Yellow box indicates portion of antrum that was taken, and blue box indicates section of corpus that was taken to derive 2D lines. (b) Representative phase contrast image of passage 6 human epithelial cells expanded in 2D culture conditions, scale bars, 100 μm. (c) Representative expression and distribution of GKN1 and MUC5AC protein in 2D culture by immunofluorescence, scale bars, 50 μm. (d) Single cell RNA sequencing on p6 human antrum and corpus 2D culture, embedded using UMAP, and clustered using Conos (Fig. 4c). Normalized heatmap showing top 20 upregulated DEGs using a representative number of cells within each cluster is shown. (e) Joint UMAP embedding of human 2D culture showing expression levels of the base/neck markers LGR5, AQP5, INSC, NFATC4, and SLC9A4.

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