Extended Data Fig. 6: Comparison of 2D-cultured cells derived from human and mouse(a) Strategy used to polarize human 2D culture. | Nature Cell Biology

Extended Data Fig. 6: Comparison of 2D-cultured cells derived from human and mouse(a) Strategy used to polarize human 2D culture.

From: Dissection of gastric homeostasis in vivo facilitates permanent capture of isthmus-like stem cells in vitro

Extended Data Fig. 6: Comparison of 2D-cultured cells derived from human and mouse(a) Strategy used to polarize human 2D culture.The alternative text for this image may have been generated using AI.

(b) Representative immunofluorescence staining of the parietal cell marker GIF in human antrum cultured using ALI, scale bar, 25 μm. (c) Correlation plot using the cumulative expression value for each gene that can be compared between species. Cumulative log expression of mouse genes are plotted on the Y-axis and the cumulative log expression of human genes are plotted on the X-axis. R was calculated using a two-sided Pearson correlation test. (d) Single cell expression of Clu in mouse primary gastric epithelial cells embedded using UMAP. (e,f,g) Human and mouse cells cultured in 2D culture conditions were jointly embedded using UMAP and clustered using Conos (Fig. 4k). Normalized heatmap showing the top 10 most shared or most differentially expressed genes between human and mouse for each cluster identified using 100 randomly selected cells from each group.

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