Extended Data Fig. 2: SARS-CoV-2 causes CHK1 and RRM2 reduction leading to cell cycle progression impairment. | Nature Cell Biology

Extended Data Fig. 2: SARS-CoV-2 causes CHK1 and RRM2 reduction leading to cell cycle progression impairment.

From: SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescence

Extended Data Fig. 2

A) IF images of infected HNEpC; nuclei were stained with Hoechst. Scale bar, 10 μm. B) Quantification of the percentage of RRM2-expressing cells in infected (SARS-CoV-2 N + ) or not (SARS-CoV-2 N-) HNEpC. C) Immunoblotting of whole cell lysates of Calu-3 infected, or not, with SARS-CoV-2 and analyzed at different time points post-infection. D) Quantification of protein levels shown in C; values are shown as relative to mock-infected samples. E,F) RT–qPCR of CHK1 and RRM2 mRNA expression in infected (V+ ) or mock-infected (V−) Huh7 and Calu-3 cells, respectively. G) Immunoblotting of CDT1 in Huh7 and Calu-3 cells treated as indicated. The experiment was repeated three times with similar results. Source numerical data and unprocessed blots are available in source data.

Source data

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