Extended Data Fig. 3: STING co-localized with TfnR-EGFP after stimulation.
a, Sting−/− MEFs stably expressing mRuby3-STING and TfnR-EGFP were imaged by Airyscan super-resolution microscopy every 1 min after stimulation with DMXAA. Selected images from the movie are shown. See also Supplementary video 1. The boxed areas in the merged images are magnified and shown. b, TfnR-EGFP (cyan) and mRuby3-STING (magenta) were stably expressed in Sting−/− MEFs. Cells were treated with DMXAA for 3 h and then with LysoTracker Deep Red (yellow). Live cell imaging was performed with Airyscan super-resolution microscopy. The boxed areas in the top panels are magnified in the bottom panels. c, The Pearson’s correlation coefficient between mRuby3-STING and TfnR-EGFP is presented in box-and-whisker plots with the minimum, maximum, sample median, and first vs. third quartiles. d, Sting−/− MEFs stably expressing TfnR-EGFP were treated with DMXAA (25 µg ml−1) or HT-DNA (4 µg ml−1) for 3 h. Cells were fixed, permeabilized, and immunostained with anti-Lamp1 antibody. The fluorescence intensity of TfnR-EGFP in Lamp1-positive compartments (Lamp1+) or in whole cell was quantified. Data are presented in box-and-whisker plots with the minimum, maximum, sample median, and first vs. third quartiles. Scale bars, 10 μm in (a, b, and d), 1 µm in the magnified images in (a, b, and d). The sample size (n) represents the number of cells (c and d). Source numerical data are available in source data.
