Extended Data Fig. 4: STING was directly encapsulated by Lamp1+ compartments.
a, EGFP-Rab5a (green) and mRuby3-STING (magenta) were stably expressed Sting−/− MEFs. Cells were treated with DMXAA for the indicated times. Cells were fixed and imaged by Airyscan super-resolution microscopy. The yellow boxed areas are magnified and shown in the right panels. b, Sting−/− MEFs stably expressing mRuby3-STING were treated with DMXAA (25 µg ml−1) as indicated. Cells were then fixed, permeabilized, and immunostained with anti-EEA1 or anti-LBPA antibody. Data are presented in box-and-whisker plots with the minimum, maximum, sample median, and first vs. third quartiles as the ratio (%) of [mRuby3-STING inside endosome]/[mRuby3-STING in whole cell]. The white boxes are magnified and shown in the bottom panels. c-e, Cells were imaged by Airyscan super-resolution microscopy every 5 seconds from 3 hours after DMXAA stimulation. The time-lapse images of the regions outlined by the yellow boxes in (c) are shown sequentially in (d) and (e). The dotted green lines indicate the limiting membrane of lysosome. Scale bars 10 μm in (a, b, and c), 500 nm in (d and e), 500 nm in the magnified images in (a and b). The sample size (n) represents the number of cells (b). Source numerical data are available in source data.
