Extended Data Fig. 7: Insulin secretion of CD63hi and CD63lo β cells in response to GLP-1 agonist.
a, Flow cytometry analysis for annexin V and 7-AAD in isolated CD63hi and CD63lo β cells from WT mice. Data are representative of 3 independent experiments (cells pooled from at least 5 mice per experiment). b, Static glucose-stimulated insulin secretion (GSIS) assay in the absence or presence of 100 μM 3-isobutyl-1-methylxanthine (IBMX). Results are normalized by insulin content in CD63hi and CD63lo murine pseudo-islets (N = 3 per group). Welch’s unpaired two-tailed t test is used for comparison. c, Venn diagram illustrating the overlap of differentially expressed genes (FDR < 0.01) between Cluster 0 β cells obtained by scRNA-seq and sorted CD63lo β cells determined through bulk RNA-seq. Biological process enrichment analysis of differentially expressed genes in both Cluster 0 and CD63lo β cells. d, Glp1r mRNA expression in sorted CD63hi and CD63lo mouse β cells determined by bulk RNA-seq. Comparisons were performed by Wald test. Benjamini–Hochberg corrected two-tiled p-value. e, Static GSIS assay in the absence or presence of 5 nM Exendin-4 (Ex4). Results are normalized by insulin content in CD63hi and CD63lo murine pseudo-islets (N = 4 replicates per group). Results are representative of 3 independent experiments. Data are presented as mean ± S.E.M. Paired two-tailed t test is used for comparison. Source numerical data are available in source data.
