Extended Data Fig. 7: Morphology of differentiating F-Cells.

(a) Top: diagram of the argos (aos) locus. Black boxes indicate exons and black lines indicate introns. Boxes below the large intron indicate the enhancer-driven GAL4 lines screened in this study. The box highlighted in magenta denotes the GAL4 line showing restricted expression in the F-Cell. Bottom: expression of mCherry under the control of 25c01-GAL4 in a Diap1-GFP background (green) showing the co-labelling of the mCherry with the cells expressing high levels of Diap1-GFP (that is, F-Cells). (b) Time course of the shape changes underlying F-Cell differentiation. The morphology of the F-Cell is marked by the expression of a membrane localized GFP under the control of 25c01-GAL4 (magenta). See Supplementary Video 3. (c) Volume rendering of the differentiating F-Cell (coronal view) coloured in magenta (top) or after applying a 5-rumps LUT scale for z-depth (bottom). Note the basal shifting of the F-Cell as the cuticle is deposited apically over time. Results are representative of three independent experiments. Scale bar is 15 μm (a) and 5 μm (b-c). Full genotypes are listed in Supplementary Table 1.