Extended Data Fig. 3: Criteria for identification of integration stage for differentiating progenitor cells.

Integration stage is assessed by localization of two key markers: (1) apical membrane, and (2) SJ. Cartoons display marker localization for Stages 0–5. Apical membrane, cyan; SJEC-EC, yellow; SJPC-EC, orange; progenitor cell (Su(H)-lacZ+ enteroblast or pre-enterocyte), blue; mature neighbor enterocytes, gray.