Extended Data Fig. 7: Top-ranking ModReg and TransReg reaction patterns identified in steady-state proteomics and metabolomics data in the mct1 Δ background.
From: Metaboverse enables automated discovery and visualization of diverse metabolic regulatory patterns
Stamp view snapshots of four of the top-ranking ModReg and four of the top-ranking TransReg reaction patterns in the mct1 Δ vs. wild-type comparison using steady-state (12-hr) proteomics and metabolomics data and sorted by associated statistical values. In cases where the substrates, products, and modifiers were identical, only the first reaction pattern is shown. Reaction patterns were sorted by difference in magnitude of the different relevant components. Only results where the input/output and modifier were both statistically significant are shown. 12-hr RNA-sequencing comparisons contained n=4 in each group, 12-hr proteomics comparisons contained n = 3 in each group, and 12-hr metabolomics comparisons contained n = 3 in each each comparison group. a.ModReg reaction #1: propionyl-CoA + carnitine = > propionylcarnitine + CoA SH, b.ModReg reaction #2: alpha-ketoadipate + CoA SH + NAD+ = > glutaryl-CoA + CO2 + NADH + H+, c.ModReg reaction #3: FXN:NFS1:ISD11:ISCU assembles 2Fe-2S iron-sulfur cluster, d.ModReg reaction #4: Succinate < = > Fumarate (with FAD redox reaction on enzyme), e.TransReg reaction #1: SLC25A16 transports cytosolic CoA SH to mitochondrial matrix, f.TransReg reaction #2: SLC25A18,A22 cotransport Glu, H+ from cytosol to mitochondrial matrix, g.TransReg reaction #4: Electron transfer from ubiquinol to cytochrome c of complex III, and h.TransReg reaction #6: Transport of Citrate from Mitochondrial Matrix to cytosol. Source numerical data are available64.
