Fig. 4: Metaboverse-identified signatures suggest compensatory mechanisms to mitochondrial dysfunction in yeast.

a, Spot growth assays for WT and mct1Δ mutant strains with EV, overexpression of CTP1 and overexpression of CTP1–GFP construct on SR-URA. b, GO term enrichment results for genes identified in the SpQN-corrected co-expression analysis of CTP1 in the refine.bio WT cohort (n = 1,248). c, Heat map of amino acids for WT and mct1Δ mutant strain proteomics at 3 h after the switch to raffinose from glucose. d, Graphical overview of yeast glycolysis pathway and other related reactions overlaid with summary annotations based on RNA-seq, proteomics and metabolomics measurements during steady-state growth (12 h). RTG, retrograde signaling pathway. A more complete legend for the shading criteria can be found in Extended Data Fig. 8. e,f, Heat maps of amino acid-regulated enzymes (e) and anaplerotic enzymes (f) for WT and mct1Δ mutant strain proteomics at 12 h after the switch to raffinose from glucose. Heat map values were mean centred at 0 (z score). Hierarchical clustering was performed where indicated by the linkage lines using a simple agglomerative (bottom-up) hierarchical clustering method (or UPGMA). Source plate images and numerical data are available at ref. ⁵⁵.