Extended Data Fig. 3: Genomic localization of PBRM1 and other PBAF subunits. | Nature Cell Biology

Extended Data Fig. 3: Genomic localization of PBRM1 and other PBAF subunits.

From: PBRM1-deficient PBAF complexes target aberrant genomic loci to activate the NF-κB pathway in clear cell renal cell carcinoma

Extended Data Fig. 3

a) PBRM1 ChIP-seq profiles of PBRM1-deficient NCC432 ccRCC cells expressing either empty vector (EV) or wild-type PBRM1. b) Genomic localization of PBRM1 binding sites in parental PBRM1-proficient cell lines (endogenous) and in PBRM1-deficient cell lines expressing wild-type PBRM1 cDNA (exogenous) annotated by ChIPseeker. c) Genomic localization of PBRM1, BRD7 and ARID2 ChIP-seq in 786-O cells shows promoter-centric occupancy of PBRM1 and BRD7 but equal distribution of ARID2 in both promoters and distal regions. d) While the majority of the PBRM1 binding sites are bound by all subunits of the PBAF subunits and SMARCA4 (canonical sites), there are also sites that are bound by PBRM1- or ARID2- deficient PBAF subcomplexes.

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