Fig. 1: Multi-omic profiling of SC-islets shows unique chromatin accessibility signatures in endocrine cell types.
a, Schematic of SC-islet differentiation and multi-omic sequencing. b, UMAPs showing identified cell types in SC-islets 2 weeks into stage 6 using both and either chromatin accessibility (ATAC) or gene (mRNA) information (29,526 cells from 3 independent differentiations; integration of all samples). c, Heat map showing gene expression of markers associated with each cell type. d, ATAC plots showing chromatin accessibility of SC-islet cell types around the INS, GCG and SST genomic regions. e, Heat map showing the top 200 variable DNA-binding motif accessibility within endocrine cell populations and highlighting markers for each cell type. f, Heat maps highlighting gene expression and ATAC motif accessibility of top ten active transcription factors co-enriched with both features in SC-β, SC-α, SC-δ and SC-EC cells. g, UMAP showing gene expression and motif accessibility of selected transcription factors associated with SC-β cells or SC-EC cells. SC, stem cell derived; EC, enterochromaffin.
