Extended Data Fig. 6: Time course characterization and analysis of long-term in vitro SC-islets.
a, Bright field images of SC-islets from week 2, week 3, week 4, month 6, and month 12 of in vitro culture. Individual image representative of 1 sample. b, Integrative UMAP of in vitro SC-islet cells including all time points. c, Heatmap showing increase, or decrease, of marker gene expression in SC-islet cells throughout time (40332 cells from 5 independent biological samples, 1 from each timepoint). d, Glucose stimulated insulin secretion assay, plotting mean ± s.e.m. (by ELISA, n = 4 biologically independent samples), of SC-islets cultured in vitro at week 2 (P = 0.020), week 3 (P = 0.0080), week 4 (P = 0.046), and month 6 (P = 0.27) time point. Statistical significances were assessed using paired two-sided t-test. e, UMAP and bar plots showing composition information of cell types from integrated time course datasets. f and g, Pearson correlation to compare all timepoints, of cell types, (f) using top 2000 most variably expressed genes (mRNA), and (g) using top 2000 most variably accessible promoters from ATAC. EC, enterochromaffin; ns, not significant.
