Extended Data Fig. 4: Ribosomes are less prominent on desmosome-adjacent ER than planar ER.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a, b) Rotated views showing that ER structures (magenta) have fewer bound ribosomes (white) at desmosome (yellow)-adjacent regions (white arrows) than at regions further away from the desmosome (blue arrows) in both top and bottom cells. Images are representative of n = 1 cell-cell contact. Scale bar = 1μm. (c–e) Three-dimensional reconstructions of segmentations in 4 × 4 × 4nm3 FIB-SEM data reveal keratin filaments (teal) contacting and penetrating planar ER sheets (magenta). Microtubules (orange) are also present in the vicinity of planar ER, and ribosomes (white) decorate the surface of ER sheets. (f–h) Rotated views of the same region in C-E. Images are representative of n = 1 cell. Scale bar = 500 nm (C-H).
