Extended Data Fig. 6: ER tubules closely associate with desmosomes in live cells.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a–e) Snapshots of a live-cell time-course of Desmoplakin-EGFP (DP) (top row, orange in bottom row) and mApple-VAPB (middle row, magenta in bottom row) in A431 cells at the cell-cell contact over 2 minutes (Images are representative of n = 3 independent experiments). Gamma correction was applied to grayscale images. Gamma (inverted) = 1.5 (VAPB). Scale bar = 2μm. (f–j) ilastik-segmentations of DP (top row) and VAPB (middle row) channels in A-E. Bottom row indicates overlapping pixels (white) between DP and VAPB channels. Overlap indicates that tips of ER tubules colocalize with DP puncta. Scale bar = 2μm.
