Extended Data Fig. 7: ER retracts from cell-free edges only in cells where microtubules are depolymerized.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a, b) Representative images of A431 cells treated with either DMSO or 30μM nocodazole reveal that ER (VAPB, magenta) tubules are converted to sheets with nocodazole treatment. The ER remains tethered to desmosomes (DP, orange) at cell-cell contacts under both treatment conditions. Images are representative of at least n = 2 independent experiments. Scale bar = 2μm. (c, d) Representative images of A431 cells treated with either DMSO or 30μM nocodazole reveal that ER (VAPB, magenta) remains close to the periphery of a cell-free edge in DMSO-treated cells (C) but retracts in nocodazole-treated cells (D). Tubulin Tracker Deep Red labelling reveals a lack of microtubules in nocodazole-treated cells but not in DMSO-treated cells. Cell-free edge is depicted by a dashed red or white line. Images are representative of at least n = 2 independent experiments. Scale bar = 2μm. Gamma correction was applied to some grayscale images. Gamma (inverted) = 1.75 (D, DP channel), 0.75 (A, Tubulin Tracker channel), 1.5 (C, Tubulin Tracker channel).
