Extended Data Fig. 2: LINC00881 contains no coding potential.
From: Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism
a, ORFs predicted in LINC00881, showing predicted peptide sequence of each ORF. A FLAG tag was inserted right after each ORF. b, Western blotting detection of peptide expressions in HEK293T cells transfected with positive control, vector, and FLAG tagged ORF1, ORF2 or ORF3. Positive control is an irrelevant FLAG tagged protein coding gene. The experiment was carried out 2 times with similar outcomes. c, Immunofluorescent staining to detect expressions of FLAG tagged peptides in HEK293T cells transfected with positive control, vector, and FLAG tagged ORF1, ORF2 or ORF3. d, RT-qPCR detecting LINC00881 RNA expression levels in HEK293T cells transfected with positive control, vector, and FLAG tagged ORF1, ORF2 or ORF3. Bars are presented as mean ± s.e.m. Unpaired two-tailed t-test is used for comparison. **p < 0.01, ***p < 0.001, (n = 3 independent experiments). Source numerical data and unprocessed blots are available in source data.
