Extended Data Fig. 3: Extended Data Figure. 3 related to Fig. 2. Single molecule analysis of H3K9me3 accumulation at stressed replication forks.
From: Dynamic de novo heterochromatin assembly and disassembly at replication forks ensures fork stability
(a, b) Left, representative chromatin fibers in the absence of treatment (a) and after a 1 hr incubation in the presence of 1 mM HU (b). Right, intensity profiles of the each representative fibers. The intensity profiles of EdU (red), H3K9me3 (green) and H3 (blue) have been plotted. These experiments were reproduced independently 3 times with similar outcomes. (c) Correlation analysis of H3K9me3 and H3 at EdU spot is shown both for untreated (left) and for cells treated with 1 mM HU for 1 hr (right). R2 indicate the correlation coefficient between H3K9me3 and H3 intensity distribution. (d) Analysis of ChromStretch fibers. Quantification of the intensity of H3, H3K9me3 and EdU both at ongoing (UT) and stalled (HU) replication forks. (nUT = 106, nHU = 104 individual replication tracks analyzed; **** = P ≤ 0.0001, ns = non-significant, One-way ANOVA). Source numerical data are available in source data.
