Fig. 5: Loss of transient H3K9me3 accumulation at stalled forks impairs replication fork stability and causes genome instability.
From: Dynamic de novo heterochromatin assembly and disassembly at replication forks ensures fork stability
a, Top: schematic of replication fork degradation assay with CldU and IdU labelling. Bottom: ratio of IdU to CldU tract length was plotted for the indicated conditions. (nsiCTL = 207, nsiBRCA1 = 214, nsiSUV39h1 = 213, nsiCTL+UNC0642 = 207, nsiBRCA1+UNC0642 = 239, nsiSUV39h1+UNC0642 = 203 replication tracks analysed; ****P ≤ 0.0001, NS, non-significant, Kruskal–Wallis test followed by Dunn’s test). b, Top: schematic of the fork restart assay. Bottom: the IdU track length (µm) was plotted to show fork restart (nWT = 150, nUNC0642 = 150, nG9aKO = 150; ****P ≤ 0.0001, NS, non-significant, Kruskal–Wallis test followed by Dunn’s test). c, Top: schematics of ssDNA gap accumulation. Bottom: the IdU track length (µm) was plotted to assess the accumulation of ssDNA behind the forks for the indicated conditions (nWT_S1− = 151, nUNC0642_S1− = 157, nG9aKO_S1− = 151, nWT_S1+ = 153, nUNC0642_S1+ = 153, nG9aKO_S1+ = 153 replication tracks analysed; ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, NS, non-significant, Kruskal–Wallis test followed by Dunn’s test). Source numerical data are available in Source Data.
