Extended Data Fig. 2: Extended Data Figure. 2 related to Fig. 1. Profiling of histone PTMs by quantitative mass spectrometry upon recovery from replication stress.
From: Dynamic de novo heterochromatin assembly and disassembly at replication forks ensures fork stability
(a) Chromosome-wide profiles of ChIP-seq data for H3K9me3 and total H3 visualized Hilbert curves. Profiles for chromosomes 1, 7 for proliferating (P), quiescent (Q) and HU-treated (RS) cells in two biological replicates are shown. (b) Experimental design (top). TIG3 cells were treated for 6 days with 600 μM HU and allowed to recover for 9 days after removal of the drug (+HU/R) or cultured in the absence of HU for the whole period (-HU). Analysis of cell proliferation by high-content live-cell imaging (bottom). The graphs show the mean confluence (%) +range from two technical replicates and are representative of two independent biological replicates. (c) Analysis by quantitative mass spectrometry of histone PTMs in single cell clones derived from proliferating cells (control) or cells allowed to recover after persistent replication stress (HU recovery). Five clones were analyzed for each condition. The graphs show the average of three technical replicates with error bars indicating SD.
