Extended Data Fig. 8: Spartin deficiency does not affect motor neuron differentiation.
From: The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets
a, Scheme of the strategy used to target TRE3G-NIL to the AAVS1 locus. b, Genotyping PCR results for iPSC-AAVS1-TRE3G-NIL cell line. c, Karyotype of iPSC-AAVS1-TRE3G-NIL cell line. d, Parental and spartin KO iPSC clones were differentiated to motor neurons in the differentiation medium for 12 days. The cells were fixed and stained with MAP2 antibody. Scare bar: 10 μm.
