Extended Data Fig. 2: STING vesicles retained in the cytosol by Baf A1 or CQ restricts aerobic glycolysis.

a, L929 cells were transfected with control siRNA or Tsg101 siRNA, and the expression of Tsg101 was quantified at 72 h post-transfection. b, L929 cells from (a) were stimulated with HT-DNA (1 μg/ml) for 8 h. The expression of Cxcl10 and Isg56 was quantified by qPCR. c, L929 cells from (a) were stimulated with HT-DNA (1 μg/ml) for 24 h, followed by lactate measurement. d, L929 cells were transfected with Control siRNA or Atp6v1b2 siRNA, and the expression of Atp6v1b2 was quantified at 72 h post-transfection. e, Sting1^−/− MLF cells stably expressing mRuby3-mSTING and Lamp1-EGFP were treated with control siRNA, Atp6v1b2 siRNA or Tsg101 siRNA for 60 h, and then stimulated with DMXAA (25 μg/ml) for 12 h. Cells were fixed and imaged by STED super-resolution microscopy. Scale bars, 5 μm. The boxed areas in the top panels were magnified in the bottom, and the fluorescence intensity profiles along the white dotted lines were shown. f, Sting1^−/− MLF cells stably expressing mRuby3-mSTING and Lamp1-EGFP were stimulated with DMXAA (25 μg/ml), and treated with brefeldin A (10 μg/ml), chloroquine (20 μM), or bafilomycin A1 (400 nM) for 12 h. Cells were fixed and imaged by STED super-resolution microscopy. Scale bars, 5 μm. The boxed areas in the top panels were magnified in the bottom, and the fluorescence intensity profiles along the white dotted lines were shown. g, immunoblotting analysis of THP-1 cells infected with HSV-1 (MOI = 1). h and i, Sting1^−/−L929 cells stably expressing FLAG-STING were transfected with HT-DNA (1 μg/ml) (h) or infected with HSV-1 (MOI = 1) (i), followed by immunoblotting. Data are presented as means ± s.d. of n = 3 independent biological replicates for a-d. P-value was calculated by two-tailed Student’s t-test. Data are representative of three independent experiments. Uncropped gel images and numerical data are available in source data.

Source data