Extended Data Fig. 7: Analysis of the asah-1 endogenous reporter.

a, Nucleotide sequence 200 bp upstream of the asah-1 start codon. Upper sequence shows the wild-type sequence highlighting putative transcription factor-binding motifs for CEH-60 (red) and PQM-1 (blue, motif). The bottom sequence shows the independent mutations introduced to delete each putative transcription factor-binding motif (yellow highlights) that introduced the following restriction enzyme recognition sequences: CEH-60 motif (NotI) and PQM-1 motif (BamHI). b, GFP–H2B expression from the asah-1::f2a::gfp::h2b endogenous reporter (rpIs176) was detected in the intestine from late embryogenesis (threefold stage) throughout larval development and into adulthood. A schematic of each animal is shown next to the fluorescence micrographs. Note that Pasah-1::NLS::gfp expression is consistently brighter in the anterior intestine (red line). Anterior to the left in the worm image. Pharynx marked by a white asterisk. Scale bar, 20 μm.