Extended Data Fig. 9: RBFOX2 functions as an m6A regulator in AML cells.
a, Average profile (top panel) and heatmap (bottom panel) showing RBFOX2 binding intensity at the m6A peak centers and the flanking 2.5 kb regions in NB4 cells. b, Average profile of RBFOX2 binding intensity at the RBFOX2 peak centers and the flanking 2.5 kb regions in NB4 cells. RBFOX2 peaks were categorized into two groups according to whether they overlap with m6A (+) or not (−). c, Distribution of m6A or RBFOX2 peaks at distinct genomic regions including promoter, exonic, intronic, transcription termination sites (TTS) and intergenic regions annotated by HOMER in NB4 cells. (+) sign represents regions harboring m6A (+) or bound by RBFOX2 (+), while the sign (−) indicates the absence of m6A (−) or RBFOX2 (−). d, Western blots of the immunoprecipitated RBFOX2 from NB4 cells and its interaction with METTL3, METTL14 and RBM15 after RNase A/T1 treatment, respectively. e, Western blots of the immunoprecipitated YTHDC1 from NB4 cells and its interaction with RBM15 after RNase A/T1 treatment. f, Western blots of the immunoprecipitated EZH2 or SUZ12 from NB4 cells, and their interactions with YTHDC1 after RNase A/T1 treatment.
