Fig. 4: RBFOX2 depletion promotes the differentiation of leukaemia cells.
a, Colony-forming assays of K562 cells transduced with control (shNS) or two RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). n = 3. b, Effects of RBFOX2 KD on K562 cell growth. n = 3. P value was calculated by two-way ANOVA, Dunnett’s multiple comparisons test. c, Flow cytometric analysis of CD61+ cell populations in K562 cells transduced with shNS or RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). d, Relative expression of CD61 in control (shNS) and RBFOX2 KD (shRBFOX2-1 and shRBFOX2-2) K562 cells. n = 3. Two-sided P value was calculated by Student’s t-test. e, Wright-Giemsa staining of cytospin slides of K562 cells transduced with shNS or RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). Arrows indicate differentiated cells. Scale bar, 40 µm. f, Flow cytometric analysis of CD61+ cell populations in control group (shNS + EV, control shRNA (shNS) with empty vector (EV)), RBFOX2 KD group (shRBFOX2 + EV, RBFOX2 KD with EV) and RBFOX2 rescue group (shRBFOX2 + RBFOX2, RBFOX2 KD with RBFOX2 overexpression) in K562 cells (left), and statistics from three biological replicates (right). EV or RBFOX2 overexpressed cells were labelled with green fluorescent protein (GFP), shNS or shRBFOX2 transduced cells expressed mCherry. GFP+mCherry+ cells represent positively double transduced cells. n = 2. g, Flow cytometric analysis of CD11b+ cell populations in control (shNS) and RBFOX2 KD (shRBFOX2-1 and shRBFOX2-2) NB4 cells. h, Relative expression of CD11b in control (shNS) and two RBFOX2 KD (shRBFOX2-1 and shRBFOX2-2) NB4 cells. n = 3. i, Wright-Giemsa staining of cytospin slides of NB4 cells transduced with shNS or RBFOX2 shRNAs (shRBFOX2-1 and shRBFOX2-2). Arrows indicate differentiated cells. Scale bar, 20 µm. n, biologically independent samples. Data are presented as mean ± standard error of the mean. For a and h, P values were calculated by one-way ANOVA, Dunnett’s multiple comparisons test.
