Extended Data Fig. 3: Treatment of already established iPS cell line with D + Q does not expand cellular plasticity.
From: p16High senescence restricts cellular plasticity during somatic cell reprogramming
a) In vivo reprograming was induced with DOX in drinking water and 3 weeks later mice were administered with D + Q by gavaging or mock-treated. Analysis of p16High cells in developed teratoma showed a strong reduction in mice treated with D + Q. Scale bar - 200 μm. b) Analysis of macrophage infiltration in teratoma of a control mTmG mouse and mouse received D + Q. Scale bars 50 μm. c) H&E staining of teratoma obtained from mouse treated with D + Q. Mesoderm: smooth muscles, SM; ectoderm: neural rosette, NR; endoderm: endodermal tubes, ET. d) qPCR analysis of line-1 retroelement (total and two subclasses: md_a and md_t) and the 2C gene, zfp352, in control iPS cells and control iPS cells treated with D + Q after iPS line was established. Error bars represent SD, p-value evaluated by unpaired, two-tailed Student’s t-test with Welch’s correction, independent repeats, n = 3.
