Extended Data Fig. 7: Removal of p16High cells improves liver plasticity during partial 4F reprogramming in aging mice.
From: p16High senescence restricts cellular plasticity during somatic cell reprogramming
a) A scheme for 4F-induced rejuvenation experiments with different mouse strains as indicated. b) Immunofluorescent analysis of DNA damage marker 53bp1 in the liver of 2-month-old mouse (mTmG) and 18-month-old mice: p16Cre;mTmG (mTmG), p16Cre;DTA (DTA), p16Cre;i4F;mTmG (i4F), p16Cre;i4F;DTA (DTA+i4F). Quantification is shown in the graph ( ± SD). Scale bar – 20 μm. c) Immunofluorescent staining for endothelial marker Cd146 in liver samples as in (B). Percentage of the area covered by Cd146+ cells is shown in the graph. Scale bar – 100 μm. d) Immunofluorescent analysis for fibrosis marker Desmin in liver samples as in (B). Scale bar – 100 μm. For panels B-D p values estimated by Brown-Forsythe and Welch ANOVA test with Dunnett multiple comparison test and only difference between i4F and DTA+i4F is shown. Data presented as mean ± SD, independent repeats, n = 3.
