Fig. 2: Su(Ste) precursor transcripts accumulate in GSCs and SGs of armi and zuc mutant testes. | Nature Cell Biology

Fig. 2: Su(Ste) precursor transcripts accumulate in GSCs and SGs of armi and zuc mutant testes.

From: A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila

Fig. 2: Su(Ste) precursor transcripts accumulate in GSCs and SGs of armi and zuc mutant testes.The alternative text for this image may have been generated using AI.

a–e, smRNA-FISH for antisense Su(Ste) precursor transcript (green) in control y1w1118 testis (a) and in piRNA pathway mutant testes of the indicated genotypes: armi mutant (b); zuc mutant (c); armi RNAi (d); zuc RNAi (e)). The corresponding magnified regions of the niche marked by quadrates are shown in a(ii), b(ii), c(ii), d(ii) and e(ii) GSC and early SGs are indicated by yellow dotted lines; cyan lines indicate zone of spermatocytes. Arrowheads point to nuclear transcripts; arrows point to cytoplasmic RNA foci. The asterisks indicate the hub. Blue, DAPI. Scale bars, 5 µm. f, Quantification of cytoplasmic Su(Ste) RNA foci in GCSs and SG cells. Signal intensity was measured by maximum projection of z-stacks that encompass the entire cell. Box plots show the median and interquartile range (IQR); whiskers denote 1.5× IQR (n = 90 for control; n = 54 for nos>armiTRIP.GL00254; n = 33 for armi1/72.1; n = 34 for nos>zucTRIP.GL00111; n = 31 for zucEY11457/−). P = 2.2 × 10−16 for Kruskal–Wallis test (one-way analysis of variance on ranks) comparing all genotypes and control; Benjamini–Hochberg-corrected P values for post hoc pairwise two-tailed Mann–Whitney tests: P = 2 × 10−16 for nos>armiTRIP.GL00254 versus control; P = 7.2 × 10−9 for armi1/72.1 versus control; P = 9.4 × 10−9 for nos>zucTRIP.GL00111 versus control; P = 2 × 10−16 for zucEY11457/− versus control. Source numerical data are available in source data.

Source data

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