Fig. 3: armi and zuc are required in GSCs and early SGs to repress Ste. | Nature Cell Biology

Fig. 3: armi and zuc are required in GSCs and early SGs to repress Ste.

From: A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila

Fig. 3: armi and zuc are required in GSCs and early SGs to repress Ste.The alternative text for this image may have been generated using AI.

a–h, Ste smRNA-FISH (red) in the testes in control y1w1118 testis (a) and in piRNA pathway mutant testes of the indicated genotypes: armi nos-driven RNAi (b); zuc nos-driven RNAi (c); armi bam-driven RNAi (d); zuc bam-driven RNAi (e); armi mutant (f); armi mutant, nos rescue (g); armi mutant, bam rescue(h). GSCs and early SGs are indicated by yellow dotted lines; cyan lines indicate zone of spermatocytes. The asterisks indicate the hub. Scale bars, 20 µm. i, Anti-Ste and anti-Tubulin western blots of whole testis lysates from the indicated genotypes. j, Male fertility of indicated genotypes (number of progeny/male/7 days). Box plots show the median and IQR; whiskers denote 1.5× IQR (n = 20 males per genotype). P < 10−5 for Kruskal–Wallis test (one-way analysis of variance on ranks) comparing all genotypes and controls; Benjamini–Hochberg-corrected P values for all post hoc pairwise two-tailed Mann–Whitney tests are shown. Source numerical data and unprocessed blots are available in source data.

Source data

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