Extended Data Fig. 1: Did2 is not detected at the SPB extrusion site, whereas Ist1 is recruited after Cmp7.
From: An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity
a, Fluorescence micrographs of Did2–GFP, mCherry–Atb2 and Pcp1–mCherry in a representative cell (MKSP3342) in anaphase B. Did2–GFP (left), sum projection of mCherry–Atb2/Pcp1–mCherry (middle) and magnifications (right) of ROI (top to bottom) green, magenta, merge. b, Plot of the percentage of nuclei with Did2–GFP (MKSP3342) co-localized with SPB marker at the indicated phases of the cell cycle. Points and error bars represent the mean and range, respectively, across at least three biological replicates with at least 50 nuclei/Did2–GFP/replicate, n = 372 nuclei. c, Time-lapse fluorescence microscopy of Cmp7–GFP (green) and Ist1–mCherry (magenta) in a late anaphase B cell (MKSP3521). Cell representative of population from two biological replicates. White boxes in overview micrographs indicate ROI magnified below images as single channel and composite images. Scale bars, 1 µm (overview) and 250 nm (ROI). Source numerical data are provided.
