Extended Data Fig. 2: A subset of PSPs are similarly localized in WT-PSs and mini-PSs.
a, Immunoblotting to examine the expression levels of various PSPs (BRG1, SFPQ, PSPC1, NONO, HNRNPH1, HNRNPF, and TDP-43) in HAP1 WT and the NEAT1 deletion mutant cells in Fig. 2a. Cells were treated with MG132 (5 μM for 6 h). GAPDH was used as a control. b, Confocal observation of the PSs and various PSPs in HAP1 WT and mini-NEAT1 mutant cells treated with MG132 (5 μM for 6 h). PSs and PSPs were visualized by RNA-FISH using NEAT1_5′ probes (green) and IF (magenta). Nuclei were stained with DAPI. Scale bar, 10 μm. c, Quantification of fluorescence intensity ratio (PSPs/NEAT1) in the HAP1 WT (blue) and mini-NEAT1 (pink) cells as observed in b (all samples: n = 30). Each box plot shows the median (inside line), 25th-75th percentiles (box bottom to top), and minimum-maximum values (whisker bottom to top). Data were compared using Mann-Whitney U-test (two-sided). If the statistical test showed no significant difference (P > 0.05), it is not specified in the figure. Source numerical data and unprocessed blots are available in source data.
