Extended Data Fig. 6: Cellular localization and expression level of YTHDF1/3.
From: O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins
(a, b) Immunoblotting of endogenous YTHDF1/3 in HEK293T, HeLa, OSMI-1 treated HEK293T and Thiamet G treated HeLa. OSMI-1: 50 µM, 5 hr; Thiamet G: 10 µM, 5 h. c, d) Quantification analysis of YTHDF1/3 expression level in (a-b). Error bars, mean ± s.d., n = 3 biologically independent repeats. Significance was calculated using two-sided t-test. The expression level of YTHDF1/3 in HeLa was lower than in HEK293T, but the inhibitors contained no effect on the expression level of YTHDF1/3. (e) Immunostaining of endogenous YTHDF1/3 in HEK293T, HeLa and Thiamet G treated HeLa. The localization of YTHDF1/3 shows not significantly different among cell lines. Scale bars: 10 μm. The experiment was repeated twice with similar results. (f) Immunostaining of Flag-tagged WT-YTHDF1/3 and YTHDF1/3-Mut in HEK293T and HeLa. The localization of YTHDF1/3 shows not significantly different among cell lines or between WT and Mut. Scale bars: 10 μm. The experiment was repeated twice with similar results.
