Extended Data Fig. 3: The selective and self-constrained nature of COPII condensation in vitro.
From: Manganese regulation of COPII condensation controls circulating lipid homeostasis
a, SEC31A/SEC13 alone doesn’t condense into protein droplets. SEC31A/SEC13 (10 μM) was assayed in 150 mM NaCl. b, Representative images of SEC23/24 droplets (10 μM) constrained with increasing SEC31A/SEC13 in 150 mM NaCl. c, Quantification of the sizes of SEC23A/SEC24A droplets in (b). n = 161, 183, 169, 117 SEC23/24 droplets for the presence of 0, 5, 10, 20 μM SEC31/13, respectively. Lines represent median; edges represent the interquartile range; whiskers represent the 5% percentile and 95% percentile. *P values are determined by one-way ANOVA test. #P values are determined by the posthoc test of Tukey. d, Size dependent exclusion of dextran by the SEC23/24 droplets. 1 μM of TAMRA-labeled dextran are mixed with 10 μM of SEC23A/SEC24A in 150 mM NaCl. Lower: relative fluorescence intensity along the dashed line. For a-d, Representative of 3 biological independent experiments is shown. Scale bars, 5μm (a, b, d). Source numerical data are available in Source Data.
