Extended Data Fig. 5: A manganese messenger that enables viral hijacking of COPII condensation.
From: Manganese regulation of COPII condensation controls circulating lipid homeostasis
a, The CP-factor in cytosol extracted from Adv infected liver is resistant to Benzonase cleavage or Proteinase K (PK) digestion. b, Representative images in Fig. 3c. Each fraction was incubated with 6 μM of SEC23A/SEC24A, followed by microscopy imaging. c, Ca2+ concentrations in cytosol extracted from CTL or Adv infected mouse liver were estimated by o-cresolphthalein complexone based colorimetric assay. n = 6 biological independent samples for each group. P values were determined by unpaired two-sided Student’s t-test. d, IB analysis of TMEM165 in mouse primary hepatocytes treated with 500 μM of indicated metal for 4 hours. e, Extracellular EDTA minimally restores TMEM165 levels in cells infected with AdV. 12 hours post infection, primary hepatocytes were treated with the indicated reagents, followed by another 12 hours-culture. f, Confocal images of endogenous SEC24A treated with or without 500 μM of MnCl2. EGFP is knocked in at the N terminus of SEC24A in Huh7 cells. g, Confocal images of endogenous SEC24A and SURF4 treated with or without 500 μM of MnCl2. For a-b and d-g, Representative of 3 biological independent experiments is shown. Scale bars, 5 μm (a-b, f-g). Source numerical data and unprocessed blots are available in Source Data.
