Extended Data Fig. 9: Mitochondrial manganese store regulates the COPII machinery for lipid control.

a, Confocal analysis of the ER (SEC61β-RFP), Golgi (anti-GM130 antibody) and lysosome (LysoTracker^TM Deep Red) in primary hepatocytes infected with or without AdV. Representative of 3 independent experiments is shown. b, Gating strategy for analyzing mitochondrial membrane potential of primary hepatocytes with indicated treatments by TMRM staining. Singlets were selected by FCS/SSC, and the boundaries of TMRM positive (TMRM⁺) was defined by the signal of non-stained primary hepatocytes. TMRM⁺ cells were used for histogram plotting in Fig. 6b. c, FIB-SEM tomography of Mitochondria-ER contact sites (MECS) in murine hepatocytes and the lipoproteins harbored in the smooth end of the ER that exhibits close contacts with the mitochondria. Representative view reconstructed by FIB-SEM from 3 C57BL/6 J mice is shown. Lipoproteins are pseudo-colored in yellow. Scale bars, 100 nm (c) or 5μm (a).