Extended Data Fig. 1: Condensation of COPII differentiates cellular transport.
From: Manganese regulation of COPII condensation controls circulating lipid homeostasis
a. FIB-SEM tomography of mouse liver revealing robust ER-Golgi transport of lipoproteins. Representative view reconstructed by FIB-SEM from 3 C57BL/6 J mice is shown. b, IB of mouse liver lysates untreated or treated with EndoH. c, Quantification of sizes of SEC24A puncta in Fig. 1b. n = 842, 900 and 989 puncta for CTL, LMAN1 and APOBN, respectively, pooled from 10 cells for each group. Representative of 3 biological independent experiments is shown. Lines represent median; edges represent the interquartile range; whiskers represent the 10% percentile and 90% percentile. P values were determined by unpaired two-sided Student’s t-test. d, Co-localization of EGFP that was knocked in the SEC24A locus (EGFPSEC24A) and endogenous SEC31A visualized by immunostaining in Huh7. Representative of 3 independent experiments is shown. e, Confocal images of EGFPSEC24A in Huh7 cells. Middle: fold of SEC24A fluorescence between puncta and diffusive cytosol. Right: sizes of EGFPSEC24A puncta. Lines represent median; edges represent the interquartile range; whiskers represent the 10% percentile and 90% percentile. n=531puncta from 5 cells. Representative of 3 biological independent experiments is shown. f, FRAP of EGFP-knock-in SEC24A coalescence in cells. 0 s indicates the time of photobleaching. Lower: quantification of FRAP. Data are shown as mean ± SEM. n = 19 puncta from 10 cells. Representative of 3 biological independent experiments is shown. g, IB of cell lysates from mouse primary hepatocytes treated with 1,6-HD for 3 hours. n = 3 biological independent experiments. h, Time-lapse confocal analysis of the ER (SEC61β-GFP) and Golgi (GM130-GFP) in primary hepatocytes treated with 1,6-HD. Representative of 3 independent experiments is shown. Scale bars, 200 nm (a), 2.5 μm (f), 5μm (d-e, h). Source numerical data and unprocessed blots are available in Source Data.
