Extended Data Fig. 4: Evaluation of mouse and human myofibers after Pax7 ablation.
From: Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells
Related to Fig. 3. a. Some individual myofibers of Pax7-ablated mdx-NSG muscles undergo hypertrophy as measured by laminin and Imaris software, N = 3. Histogram shows mean ± SEM frequency/mm2 in 500μm2 increments;†indicates myofiber sizes found only in Pax7-DTX mice. b. Representative images of engrafted human fetal SMPCs (10X) and adult SCs (20X) and into mdx-NSG and mdx-NSG Pax7-DTX mice show increased formation of small, human-only myofibers in mdx-NSG Pax7-DTX mice as measured by human Dystrophin+ Lamin A/C+, N = 4. c. Tibialis anterior muscles of Pax7 ablated mice are atrophic. Whole muscle cross sectional area (CSA) is decreased compared to non-ablated control (cntl) mice. Graph shows mean ± SD, * = P<0.018, N = 5. H&E staining of Pax7-ablated and control mice 30 days post engraftment of 1 × 106 fetal SMPCs does not rescue weight loss, N = 5/mice, * = P = 0.025.
