Extended Data Fig. 4: Quantification of tumor size in FIRE-WT and FIRE-KO animals.
From: Microglia promote anti-tumour immunity and suppress breast cancer brain metastasis
a IVIS images show EO771 luciferase luminescence signal change over time in FIRE-WT and FIRE-KO animals. Representative animals that displayed continuous signal increase (tumor growth, solid line) vs. signal decrease (tumor rejection, dashed line) are shown. Pseudocoloring of luminescence shows quantification of radiance (p/sec/cm2/sr). b Line graphs show quantification of luminescence signal change over time in all FIRE-WT and FIRE-KO animals. Solid lines indicate animals that demonstrated tumor growth and dashed lines indicate those that showed tumor rejection. Growth was defined by signal increase over time, and rejection was defined as either baseline signal (<106) or >5-fold decrease in signal relative to maximum. c Serial dilution analysis of EO771 cell engraftment in FIRE-WT and FIRE-KO animals. 10–200 × 104 EO771 cells were transplanted intracranially into each mouse strain. Ex vivo whole brain luminescence images show signal from tumor cells in each tissue at day 14. Fractions denote the number of grafts that produced macroscopic tumors in each condition. d Dot plots quantify luminescent signal (total flux) from each tissue shown in Extended Data Fig. 4c at day 14. P values were generated by an unpaired two-sided student’s t-test.
