Fig. 1: Primary basal and luminal prostate cells have distinct metabolic features.
a, Schematic of RNA-seq, metabolic profiling and glucose tracing performed on primary basal and luminal cells isolated from mouse prostate. b, GSEA of all KEGG, Hallmark and Reactome metabolism gene sets in basal and luminal cells. c, Heatmap of glycolytic and TCA cycle enzymes from RNA-seq of three biological replicates of basal and luminal cells. d, Heatmap of metabolite abundance in primary basal and luminal mouse prostate cells with three technical replicates for each of the three biological replicates. e, Aconitate-to-citrate fractional contribution ratio in primary basal and luminal mouse prostate cells fed [U-13C]glucose tracer for 16 h. f,g, Heatmaps of genes involved in de novo lipogenesis (f) and zinc transport (g) from RNA-seq of primary basal and luminal mouse prostate cells. h,i, Percentage M2 citrate (h) and percentage M3 citrate (i) from [U-13C]glucose in basal and luminal cells (n = 3 technical replicates for each of the 3 biological replicates). j, Fold change in glycolytic and TCA cycle enzymes from RNA-seq of basal and luminal cells from three human prostates. Shaded grey rectangles indicate genes that have statistically significant (P < 0.05) differential abundance. For all panels, data are shown as mean ± s.e.m. P values were calculated using a paired two-tailed t-test. Bas, basal; Bio rep, biological replicate; Lum, luminal.
