Extended Data Fig. 7: Characterization of ER and MAF chromatin binding regions.

a, Position-weight matrices of two described MAF binding motifs obtained from the MatBase database (Genomatix software). MARE, MAF response element (half sites) (left). Prediction of MAF binding sites in the vicinity of ER ChIP-seq peaks identified in MAF-overexpressing cells after E2 stimulation generated with the MatInspector program from Genomatix. The number of input sequences with at least one match of the MARE or MAF matrix, the total number of matches in all input sequences, the expected match numbers in an equally sized sample of the genome and its standard deviation, the overrepresentation and the Z-score are shown. A Z-score below –2 or above 2 can be considered statistically significant. b, Boxplots showing enrichment of p300, a marker of active enhancers, in ERbs identified in control (left) and MAF-overexpressing MCF7 cells (right) after E2 administration. Boxes represent the first, second (median) and third quartiles. Whiskers indicate maximum and minimum values with outliers being excluded. n= number of peaks (3380 in Mock E2, 5264 in MAF E2) from 1 biological replicate per condition. c, Percentage of ER ChIP-seq peaks and MAF ChIP-seq peaks annotated according to promoter-TSS / intergenic / gene body.