Extended Data Fig. 1: Breast tumors remotely rewire HSPC differentiation towards myelopoiesis in various breast cancer models.
a, Number of EdU+ cycling LSK cells in the BM of WT (n = 6) or PyMT mice (n = 6, pool of 2 experiments). b, Lin−Sca1+Kit+ cell frequency and cell number in tumor-free (n = 9) or Py230 tumor-bearing mice (n = 9, pool of 2 experiments) and Neu mice (n = 6, one experiment). c, Representative pictures of femurs from tumor-free (n = 3) and Py230 (n = 3) or Neu mice (n = 3). d, e, Representative tissue histology of humerus sections from WT (d) and PyMT mice (e) stained with hematoxylin and eosin (performed twice on 2 WT and 2 PyMT mice). Epiphysis of humerus are a 4.6x zoom with 200μM scale bar. 40x zoom with a 20μM scale bar images from the endosteum and the BM of the same bone are also depicted. f, Total count of live cells in the BM of WT (n = 10) and PyMT mice (n = 11, pool of 4 experiments). g, Correlation between tumor weight and LSK cell number in the BM of PyMT (n = 9, pool of 3 experiments) and Py230 tumor-bearing mice (n = 7, pool of 2 experiments). h, i, Number of LSK cells at different tumor development stages in PyMT (h) or Py230 tumor-bearing mice (i). j, Cell number of CD45+Lin(CD19, NKp46, CD3, B220)- myeloid and Lin− lymphoid cells in the blood of tumor-free (n = 7), Py230 tumor-bearing (n = 7, pool of 2 experiments), WT (n = 5) and Neu (n = 4, one experiment) mice. k, l, Blood cell number of Lin-CD11b+Ly6G+ Neutrophils (k) and CD11b+Ly6G−Ly6CHi Monocytes (l) in WT (n = 7) and PyMT (n = 8, pool of 3 experiments), tumor-free (n = 7) and Py230 tumor-bearing mice (n = 7, pool of 2 experiments) or WT (n = 5) and Neu mice (n = 4, one experiment). m, n, Cell number of CD45+Lin(CD19, NKp46, CD3, B220)- myeloid and Lin− lymphoid cells in the blood of WT and PyMT mice (n = 6, pool of 2 experiments) (m) or tumor-free (n = 9, pool of 3 experiments) and Py230 tumor-bearing (n = 5 for 100mm3 and n = 3 for 500mm3 and 1500mm3) (n) at different tumor development stages. Data are the mean ± s.d. P value < 0.05 are depicted and were obtained using two-tailed t-test (a, b, f, k and l) corrected for multiple comparison using Holm-Šídák method (h, j and m), one-way Anova (i), two-way Anova (n) or simple linear regression (g).
