Extended Data Fig. 3: B12 reduces cholinergic signalling especially under conditions of increased acetylcholine release.
From: Vitamin B12 produced by gut bacteria modulates cholinergic signalling
a, Reversal frequency of unc-2(gof) and cat-1(ok411); unc-2(gof) mutants fed OP50 ± 64 nM B12 (mean ± s.e.m., two-way ANOVA with Tukey’s multiple comparison). b, Quantification of locomotion speed of wild-type and ace-1(p1000); ace-2(g72) mutants fed OP50, OP50 with 64 nM B12, Comamonas, or Comamonas cbiAΔ for 24 h (mean ± s.e.m., n = 4, two-way ANOVA with Tukey’s multiple comparison). c-f, Quantification of reversal frequency (c), locomotion speed (d), head bending (e), and body bending (f) of wild-type and unc-2(gof) mutants fed OP50 ± 64 nM B12 for 24 h (mean ± s.e.m., two-way ANOVA with Tukey’s multiple comparison). g, Quantification of paralysis percentage of wild-type animals fed OP50 on 1 mM aldicarb-containing NGM agar plates or M9 liquid buffer (mean ± s.e.m., n = 4 (crawling on agar), n = 5 (swimming on liquid), two-way ANOVA with Tukey’s multiple comparison). Source numerical data are provided.
