Extended Data Fig. 7: A neuronal choline transporter is required to mediate the effect of B12 on excitatory transmission.
From: Vitamin B12 produced by gut bacteria modulates cholinergic signalling
a, Reversal frequency of unc-2(gof), acr-23(ok2804); unc-2(gof), or lgc-41(sy1494) unc-2(gof) mutants fed OP50 ± 64 nM B12 for 24 h (mean ± s.e.m., two-way ANOVA with Tukey’s multiple comparison). b, Reversal frequency of unc-2(gof) or deg-3(u701); unc-2(gof) mutants fed OP50 ± 64 nM B12 for 24 h (mean ± s.e.m., two-way ANOVA with Tukey’s multiple comparison). c, Reversal frequency of unc-2(gof) mutants fed OP50, OP50 with B12 (64 nM), OP50 with betaine (75 mM), or OP50 with both B12 and betaine (mean ± s.e.m., two-way ANOVA with Tukey’s multiple comparison). d, Growth rate as indicated by body length of unc-2(gof) and cho-1(tm373); unc-2(gof) mutants fed OP50 ± 64 nM B12 for 24 h (mean ± s.e.m., n = 3, two-way ANOVA with Tukey’s multiple comparison). Source numerical data are provided.
