Extended Data Fig. 7: Ablation of EPAC1 in PDGFRα+ cells during diet-induced obesity.
From: EPAC1 enhances brown fat growth and beige adipogenesis
Epac1fl/fl PdgfraCre/+ (EPAC1PKO) and Epac1fl/fl Pdgfra+/+ (WT) were fed a HFD for 12 weeks. a, Food intake per day in grams n = 9 or a CD, n = 6 independent animals. b, WATg mass per body weight of HFD mice, n = 9 biologically independent samples (left panel) and representative macroscopic picture (right panel), n = 3 biologically independent samples. Scale bar 1 cm. c, BAT mass per body weight of HFD mice, n = 9 biologically independent samples. d, Representative H&E staining of BAT HFD. Scale bars, 100 µm (overview) and 50 µm (magnifications), n = 3 biologically independent samples. e, Average oxygen consumption standardized to BW (ANCOVA) of CD mice at 23 °C, n = 6 independent animals. f, ANCOVA analysis of oxygen consumption per BW after 1 h 4 °C of mice fed a CD, n = 6 independent animals. P values determined by unpaired two-tailed t-test. In a, one-way ANOVA (Tukey’s multiple comparison test). Data are presented as mean values ± SEM. Source numerical data are available in source data.
