Extended Data Fig. 9: Activation of EPAC1 induces hiPSC-BAP proliferation and differentiation.
From: EPAC1 enhances brown fat growth and beige adipogenesis
a, Representative immunoblot (left panel) and quantification (right panel) of UCP1 in hBA treated with or without 007, n = 3, biologically independent samples. b, Relative Ki67 expression of human induced pluripotent stem cells derived brown-like adipose progenitors (hiPSC-BAP) organoids stimulated with or without 007, n = 3 independent experiments. c, hiPSC-BAPs stimulated with or without 007. Proliferating cells labelled with EdU (green). DNA (blue) stained with DAPI. Green cells show EdU/DAPI-positive cells, n = 4 independent experiments. d, hiPSC-BAPs differentiated in the presence or absence of 007 from d-1 to d24, representative images of Perilipin1 (red), UCP1 (green) and DAPI (blue) staining (left panel) and quantification of relative UCP1 expression (right panel), n = 4 independent experiments. P values determined by unpaired two-tailed t-test. Data are presented as mean values ± SEM. Source numerical data and unprocessed blots are available in source data.
