Fig. 4: Ablation of EPAC1 in PDGFRα+ cells exacerbates the effects of DIO.
From: EPAC1 enhances brown fat growth and beige adipogenesis
a–k, EPAC1PKO and WT (Rapgef3fl/flPdgfra+/+) mice were fed an HFD for 12 weeks. a, Body weight over the course of 12 weeks. n = 9 (HFD) and n = 6 (CD) independent animals. b, Blood glucose over the course of 120 min (left) and AUC quantification (right). n = 9 (HFD) and n = 6 (CD) independent animals. c, Fat mass per body weight. n = 9 independent animals. d, The body composition of the HFD-fed groups. n = 9 independent animals. e, The WATi mass per body weight of HFD-fed mice (n = 9 biologically independent samples; left) and representative macroscopy image (right). Scale bar, 1 cm. n = 3 biologically independent samples. f, Representative H&E immunostaining of WATi after feeding on an HFD. Scale bars, 100 µm (overview) and 50 µm (magnifications). n = 3 biologically independent samples. g, The average adipocyte area of WATi after feeding on an HFD. n = 6 (WT) and n = 7 (EPAC1PKO) biologically independent samples. For the box plots, the centre line shows the median, the box limits show the first and third quartiles, and the upper and lower whiskers extend from the maximum to the minimum value. h, The relative frequency of adipocyte size of the WATi of HFD-fed mice. n = 6 (WT) and n = 7 (EPAC1PKO) biologically independent samples. i, Representative immunoblot (right) and quantification (left) of UCP1 from BAT after 12 weeks feeding on an HFD. Expression data were normalized to the total BAT weight. n = 6 (WT) and n = 7 (EPAC1PKO) biologically independent samples. j,k, Analysis of covariance (ANCOVA) analysis of the oxygen consumption per body weight of mice that were exposed to 23 °C (j) and 1 h at 4 °C (k) and fed an HFD. n = 9 independent animals. P values were determined using unpaired two-tailed t-tests (a, e, g and i) and one-way ANOVA with Tukey’s multiple-comparison test (b and c). Data are mean ± s.e.m. from biologically independent animals. Source numerical data and unprocessed blots are provided as source data.
