Extended Data Fig. 8: Impact of changes in Pol III composition on tRNA gene expression during differentiation.
From: Selective gene expression maintains human tRNA anticodon pools during differentiation
a, Gene expression heatmaps for POLR3G and POLR3GL in hiPSC, NPC, neurons and CM (n = 2). Scale represents standardized Z score calculated using RNA-Seq raw gene counts across samples. b, Immunoblots of RPC7α in hiPSC, NPC, neurons, and CM (n = 3 biological replicates). c–e, Venn diagrams depicting overlap between consensus RPC7α ChIP-Seq tRNA peaks in kucg-2 hiPSC and (c) consensus RPC1 tRNA ChIP-Seq peaks in kucg-2 hiPSC, (d) housekeeping tRNA genes, and (e) tRNA genes repressed during differentiation. f, Immunoblot of RPC7α hiPSC CRISPRi cells carrying a sgRNA against POLR3G (n = 2 biological replicates). Gene knockdown was induced by addition of 2 µM doxycycline for 2 d. g, MA plot generated by DiffBind of spike-in normalized RPC1 counts over tRNA features (±125 bp) vs. log2 fold-change for (f) doxycycline-induced hiPSC carrying a sgRNA against POLR3G relative to uninduced controls (n = 2 biological replicates). Grey dots: not significant (FDR-adjusted P > 0.05). Source numerical data and unprocessed blots are provided.
