Extended Data Fig. 5: Detailed analysis of EM images.
From: FOXO1-mediated lipid metabolism maintains mammalian embryos in dormancy
(a) Example masking of the embryo (yellow), nucleus (light blue), LDs (green), and mitochondria (red). Scale bar: 2 µm. (b) Masking of the zone of influence to identify the number of mitochondria in close proximity to each LD or a cluster of LDs. Masked are the nucleus (yellow), the LDs (red), a watershed around the lipid droplets (orange), the zone of interest of 80 pixels around the watershed object containing the lipid droplets (purple), and the mitochondria in the zone of interest (green). Scale bar: 2 µm. (c) Number of analyzed cells per embryo, as quantified using the EM images. n = number of embryos per condition. Data are presented as median +/- standard deviation. Statistical test is student’s T-test, two-sided.
