Fig. 5: Carnitine supplementation reduces global genomic and metabolic activity.
From: FOXO1-mediated lipid metabolism maintains mammalian embryos in dormancy
a,b, IF staining of H4K16ac and OCT4 in E4.5, mTORi (a) and mTORi + c embryos (b). Embryos (n = 8–12 per condition) were paused for 3, 8 or 15 days and or reactivated (R) for 12–24 h in fresh medium without mTORi. For each condition, a representative z-projection and single z-stack channels are shown. The ICM is highlighted with dashed white lines. Scale bar, 50 µm. c,d, Quantification of DAPI-normalized H4K16ac intensities in the ICM (OCT4-positive cells (c)) or TE (d) in each condition. n is the number of cells used for quantification. A total of 8–12 embryos were used. Plots show median, *P < 0.05, one-way ANOVA and Dunnett’s post hoc test. e, Mean-centred log2FC of metabolites detected in single embryo MALDI imaging metabolomics analysis (n = 4–12 embryos per condition per metabolite). f, An example image of the single embryo MALDI imaging, showing the relative intensity of two exemplary metabolites in green and purple. Scale bar, 100 µm. g, ESC and TSC derivation efficiency of E3.5 and D12 + c embryos. Representative bright field images of ESC and TSC colonies are shown on the right. Scale bar, 100 µm. h, Stemness marker expression in ESCs and TSCs. Left: alkaline phosphatase (AP) activity (scale bar, 100 µm) and IF for OCT4 and CDX2 are shown (scale bar, 50 µm). Two biological replicates were performed. Right: area-normalized intensity of OCT4 and CDX2.
